This web site contains research data from the U. S. Department of Energy's ENIGMA project.
This data is described in the publication: "A Computational Framework for Proteome-Wide Pursuit and Prediction of
Metalloproteins using ICP-MS and MS/MS Data".
Detailed data for each protein discussed in this publication can be viewed by clicking on the corresponding
link below. A separate chart is displayed for each column in which the protein was
detected. The number of peptides detected for each fraction within the column
are indicated by yellow bars. Peptide counts are displyed even if only a single peptide was detected, however only fractions
with two or more detected peptides contribute to the GMPA score. Metal
concentration plots for any of the ten metals detected can be viewed by clicking
the links at the top of each protein detail page. Backgound information on the
computational and experimental methods used to generate and analyze the data can
be found in the following publications:
A Computational Framework for Proteome-Wide Pursuit and Prediction of
Metalloproteins using ICP-MS and MS/MS Data
Lancaster WA, Praissman JL, Poole FL II,
Cvetkovic A, Menon AL, Scott JW, Jenney FE Jr.,
Thorgersen MP, Kalisiak E, Apon JV, Trauger SA, Siuzdak G,
Tainer TA and Adams MWW. BMC Bioinformatics (submitted)
Microbial metalloproteomes are largely uncharacterized
Cvetkovic A, Menon AL, Thorgersen MP, Scott JW, Poole FL II, Jenney FE Jr,
Lancaster WA, Praissman JL, Shanmukh S, Vaccaro BJ, Trauger SA, Kalisiak E, Apon
JV, Siuzdak G, Yannone SM, Tainer JA, Adams MWW.
Nature. 2010 Aug 5;466(7307):779-82.
Novel multiprotein complexes identified in the hyperthermophilic archaeon
Pyrococcus furiosus by non-denaturing fractionation of the native proteome
This work conducted by ENIGMA was supported by the Office of Science, Office of
Biological and Environmental Research, of the U. S. Department of Energy under
Contract No. DE-AC02-05CH11231.
Menon AL, Poole FL II, Cvetkovic A, Trauger SA, Kalisiak E, Scott JW, Shanmukh
S, Praissman JL, Jenney FE Jr, Wikoff WR, Apon JV, Siuzdak G, Adams MWW.
Mol Cell Proteomics. 2009 Apr;8(4):735-51.